Recombinant human PP2C gamma/PPM1G protein
PPM1G (Protein phosphatase 1G) is a member of the PP2C family of Serine/threonine protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The human Ser/Thr phosphatase PPM1G promotes spliceosome assembly. This phosphatase is found to be responsible for the dephosphorylation of Pre-mRNA splicing factors, which is important for the formation of functional spliceosome. Recombinant human PPM1G, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.
Product Specifications
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
27 kDa (250aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Marc Flajolet., et al. (2004) . Proc. Natl. Acad. Sci. 100 (26) :16006-11.; ; Suh EJ., et al. (2007) . FEBS Lett. 580 (26) :6100-4.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 1mM EDTA, 2mM beta-mercaptoethanol, 20% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002698
Uniprot Accession Number
O15355
Species
Human
AA Sequence
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