Recombinant human Cyclophilin F/PPIF protein
Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Molecular Weight
21.1 kDa (199aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Bergsma DJ., et al. (1991) J Biol Chem. 266 (34) :23204-14.; ; Bowles KR., et al. (2000) Genomics 67 (2) :109-27.
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_005720
Uniprot Accession Number
P30405
Species
Human
AA Sequence
Frequently Asked Questions
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