Recombinant human Cyclophilin F/PPIF protein

Cyclophilin F (also known as Peptidylprolyl isomerase F, PPIF) is a member of peptidyl-prolyl cis-trans isomerase family. They are highly-conserved cytoplasmic enzymes that accelerate protein folding. This protein is part of the mitochondrial permeability transition pore in the inner mitochondrial membrane. Activation of this pore is thought to be involved in the induction of apoptotic and necrotic cell death. Recombinant PPIF protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Product Name Alternative

Peptidylprolyl isomerase F, Peptidyl-prolyl cis-trans isomerase F mitochondrial, PPIase F, Cyclophilin D, CyP-D, CypD, Cyclophilin F, Mitochondrial cyclophilin, CyP-M, Rotamase F

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 900 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.

Molecular Weight

21.1 kDa (199aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin D, Cyclophilin F, Cyp D, CYP3, CypD, Cyp-D, hCyP3, mitochondral, Peptidyl prolyl cis trans isomerase, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F (cyclophilin F), Peptidylprolyl isomerase F Cyclophilin 3, PPIase, PPIF, Rotamase, PPF0901-10 µg, PPF0901-20 µg, PPF0901-50 µg, PPF0901-100 µg, PPF0901-250 µg, PPF0901-500 µg, PPF0901-1 mg, PPF0901-010, PPF0901-020, PPF0901-050, PPF0901-100, PPF0901-250, PPF0901-500, PPF0901-01M

References & Citations

Bergsma DJ., et al. (1991) J Biol Chem. 266 (34) :23204-14.; ; Bowles KR., et al. (2000) Genomics 67 (2) :109-27.

Other References

Gallay PA, et al. Characterization of the Anti-HCV Activities of the New Cyclophilin Inhibitor STG-175. (PLoS One. 2016) {https://pubmed.ncbi.nlm.nih.gov/27104614/}; ; Kon N, et al. A small-molecule DS44170716 inhibits Ca 2+-induced mitochondrial permeability transition. (Sci Rep. 2017) {https://pubmed.ncbi.nlm.nih.gov/28634393/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_005720

Uniprot Accession Number

P30405

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MCSKGSGDPS SSSSSGNPLV YLDVDANGKP LGRVVLELKA DVVPKTAENF RALCTGEKGF GYKGSTFHRV IPSFMCQAGD FTNHNGTGGK SIYGSRFPDE NFTLKHVGPG VLSMANAGPN TNGSQFFICT IKTDWLDGKH VVFGHVKEGM DVVKKIESFG SKSGRTSKKI VITDCGQLS