Recombinant human Cyclophilin E/PPIE protein

Cyclophilins possess the activity of peptidyl-prolyl cis-trans isomerase (PPIase) and are involved in cellular protein folding and protein interactions. PPIE contains two RNA binding domains at the N-terminal region and a PPIase domain at the C-terminal region. It was not known what cellular and physiological roles of PPIE. Recombinant human PPIE was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 210 nmol/min/ug, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Product Name Alternative

Peptidylprolyl isomerase E isoform 1, CYP-33, Cyclophilin E, PPIase E, Rotamase E, peptidylprolyl isomerase E (cyclophilin E), PPIE, Peptidylprolyl isomerase E isoform 1 Cyclophilin 33, CYP33, EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 210 nmol/min/mg, and is defined as the amount of enzyme that cleaves 1nmole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin.

Molecular Weight

37.5 kDa (337aa) confirmed by MALDI-TOF

Additionnal Information

Cyclophilin E, PPIE, Peptidylprolyl isomerase E isoform 1, CYP-33, Cyclophilin E, PPIase E, Rotamase E, peptidylprolyl isomerase E (cyclophilin E), PPIE, Peptidylprolyl isomerase E isoform 1 Cyclophilin 33, CYP33, EC 5.2.1.8 Peptidyl prolyl cis trans isomerase E, PPE0901-10 µg, PPE0901-20 µg, PPE0901-50 µg, PPE0901-100 µg, PPE0901-250 µg, PPE0901-500 µg, PPE0901-1 mg, PPE0901-10, PPE0901-20, PPE0901-50, PPE0901-100, PPE0901-250, PPE0901-500, PPE0901-1

References & Citations

Wang Y., et al. (2008) FEBS Lett. 582 (5) :835-9.; ; Mi H., et al. (1996) FEBS Lett. 398:201-5.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0)

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_006103

Uniprot Accession Number

Q9UNP9

Species

Human

AA Sequence

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMATT KRVLYVGGLA EEVDDKVLHA AFIPFGDITD IQIPLDYETE KHRGFAFVEF ELAEDAAAAI DNMNESELFG RTIRVNLAKP MRIKEGSSRP VWSDDDWLKK FSGKTLEENK EEEGSEPPKA ETQEGEPIAK KARSNPQVYM DIKIGNKPAG RIQMLLRSDV VPMTAENFRC LCTHEKGFGF KGSSFHRIIP QFMCQGGDFT NHNGTGGKSI YGKKFDDENF ILKHTGPGLL SMANSGPNTN GSQFFLTCDK TDWLDGKHVV FGEVTEGLDV LRQIEAQGSK DGKPKQKVII ADCGEYV