Recombinant human PGP9.5/UCHL1 protein

PGP9. 5/uCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9. 5 is a component of the ubiquitin system, which has a fundamental role in regulating various biological activities. PGP9. 5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson's disease susceptibility. Recombinant PGP9. 5 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 150 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

Product Name Alternative

Ubiquitin C-terminal hydrolase L1, Ubiquitin carboxyl-terminal esterase L1, Ubiquitin thiolesterase L1, Parkinson Disease 5, PARK5, PGP9.5, Uch-L1, UCHL-1, Neuron cytoplasmic protein 9.5

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 150pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0pmole of ubiquitin-AMC per minute at pH 7.5, at 37C.

Molecular Weight

24.8 kDa (223aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

UCH-L1, PGP9.5, ubiquitin carboxyl-terminal esterase L1, uCHL1, PGP9.5, 1-223aa, uCH-L1, EC 3.4.19.12, ubiquitin thioesterase L1, Neuron cytoplasmic protein 9.5, PGP 9.5, PGP9.5, ubiquitin carboxyl-terminal hydrolase isozyme L1, ubiquitin carboxyl-terminal esterase L1 Gracile axonal dystrophy, Neuron cytoplasmic protein 9.5, Park 5, Parkinson Disease 5, PGP95, uCH L1, Protein gene product 9.5, ubiquitin C terminal esterase L1, ubiquitin C terminal hydrolase (neuron specific), ubiquitin carboxyl terminal esterase L1, ubiquitin carboxyl terminal hydrolase isozyme L1, ubiquitin thiolesterase, ubiquitin thiolesterase L1., PGP0701-10 µg, PGP0701-20 µg, PGP0701-50 µg, PGP0701-100 µg, PGP0701-250 µg, PGP0701-500 µg, PGP0701-1 mg, PGP0701-10, PGP0701-20, PGP0701-50, PGP0701-100, PGP0701-250, PGP0701-500, PGP0701-1

References & Citations

Sakurai M., et al. (2006) Cell 119, 162-171.; ; Liu Y., et al. (2002) Cell. 111 (2) :209-18.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA

Scientific Category

Neuroscience

NCBI Accession Number

NP_004172

Uniprot Accession Number

P09936

Species

Human

AA Sequence

MQLKPMEINP EMLNKVLSRL GVAGQWRFVD VLGLEEESLG SVPAPACALL LLFPLTAQHE NFRKKQIEEL KGQEVSPKVY FMKQTIGNSC GTIGLIHAVA NNQDKLGFED GSVLKQFLSE TEKMSPEDRA KCFEKNEAIQ AAHDAVAQEG QCRVDDKVNF HFILFNNVDG HLYELDGRMP FPVNHGASSE DTLLKDAAKV CREFTEREQG EVRFSAVALC KAA