Recombinant human PCNA protein

PCNA (Proliferating Cell Nuclear Antigen) is found in the nucleus and is a cofactor of DNA polymerase delta. This protein is associated with DNA synthesis and repair. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. It appears during late G1- phase, S-phase of mitosis and persists until the end of the M-phase because of its long biological half-life. PCNA may be induced by uV irradiation, growth factors and eventually by neighbouring tumours. Recombinant human PCNA, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

28.7 kDa (261aa) confirmed by MALDI-TOF

Additionnal Information

PCNA, Proliferating cell nuclear antigen, MGC8367, Proliferating cell nuclear antigen, Proliferating cell nuclear antigen, PCNA, PDN0801-10 µg, PDN0801-20 µg, PDN0801-50 µg, PDN0801-100 µg, PDN0801-250 µg, PDN0801-500 µg, PDN0801-1 mg, PDN0801-10, PDN0801-20, PDN0801-50, PDN0801-100, PDN0801-250, PDN0801-500, PDN0801-1

References & Citations

Matsumoto K., et al. (1987) EMBO J. 6 (3) :637-42. ; ; Moldovan GL., et al. (2007) . Cell. 129 (4) :665-79.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA, 20% glycerol

Scientific Category

Cell Cycle

NCBI Accession Number

NP_002583.1

Uniprot Accession Number

P12004

Species

Human

AA Sequence

MFEARLVQGS ILKKVLEALK DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S