Recombinant human PDH kinase 1/PDK1 protein

PDK1 (pyruvate dehydrogenase kinase isoform 1) is involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase (PDH) that is a part of a mitochondrial multienzyme complex to catalyze the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. PDK1 has been found to serve as an effective therapeutic target for inhibition of glioblastoma growth. Recombinant human PDK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Pyruvate dehydrogenase kinase 1, Pyruvate dehydrogenase kinase, PDHK1, Isozyme 1, Isoenzyme

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

48.6 kDa (429aa)

Additionnal Information

Pyruvate dehydrogenase kinase isozyme 1, Pyruvate dehydrogenase kinase isoform 1, Pyruvate dehydrogenase kinase 1, Pyruvate dehydrogenase kinase, PDK1, PDK 1, PDHK1, PDHK 1, Mitochondrial pyruvate dehydrogenase kinase isoenzyme1, Isozyme1, Isozyme 1 HGNC:8809, Isozyme 1, Isoenzyme1, Isoenzyme 1, PDK0904-10 µg, PDK0904-20 µg, PDK0904-50 µg, PDK0904-100 µg, PDK0904-250 µg, PDK0904-500 µg, PDK0904-1 mg, PDK0904-010, PDK0904-020, PDK0904-050, PDK0904-100, PDK0904-250, PDK0904-500, PDK0904-01M

References & Citations

Gudi R ., et al. (1995) . J Biol chem. 270 (48) : 28989-94; ; Xie Z., et al. (2006) . BMC Cancer. 6 :77;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.0) containing 100 mM NaCl, 0.5mM DTT, 0.1mM EDTA, 0.1mM PMSF, 1mM MgCl2, 40% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002601

Uniprot Accession Number

Q15118

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MSSDSGSSPA SERGVPGQVD FYARFSPSPL SMKQFLDFGS VNACEKTSFM FLRQELPVRL ANIMKEISLL PDNLLRTPSV QLVQSWYIQS LQELLDFKDK SAEDAKAIYD FTDTVIRIRN RHNDVIPTMA QGVIEYKESF GVDPVTSQNV QYFLDRFYMS RISIRMLLNQ HSLLFGGKGK GSPSHRKHIG SINPNCNVLE VIKDGYENAR RLCDLYYINS PELELEELNA KSPGQPIQVV YVPSHLYHMV FELFKNAMRA TMEHHANRGV YPPIQVHVTL GNEDLTVKMS DRGGGVPLRK IDRLFNYMYS TAPRPRVETS RAVPLAGFGY GLPISRLYAQ YFQGDLKLYS LEGYGTDAVI YIKALSTDSI ERLPVYNKAA WKHYNTNHEA DDWCVPSREP KDMTTFRSA