Recombinant human PDCD4 (S48Y) protein

PDCD4 (Programmed cell death 4) encodes a tumor suppressor protein whose expression is lost in progressed carcinomas of lung, breast, colon, and prostate. The expression of PDCD4 gene is strongly induced during apoptosis in a number of cell types. It is modulated by cytokines in natural killer and T cells. Recombinant human PDCD4, was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

51 kDa (469aa) confirmed by MALDI-TOF

Additionnal Information

PDCD4 (S48Y), Programmed cell death 4 isoform 1, neoplastic transformation inhibitor, H731, MGC33046, MGC33047, Programmed cell death 4 isoform 1, PDPC4, Programmed cell death 4 isoform 1, PDCD4, programmed cell death 4 (neoplastic transformation inhibitor), nuclear antigen H731, PDC0801-10 µg, PDC0801-20 µg, PDC0801-50 µg, PDC0801-100 µg, PDC0801-250 µg, PDC0801-500 µg, PDC0801-1 mg, PDC0801-10, PDC0801-20, PDC0801-50, PDC0801-100, PDC0801-250, PDC0801-500, PDC0801-1

References & Citations

Yoshinaga H., et al. (1999) Pathol Int. 49 (12) :1067-77.; ; Palamarchuk A., et al. (2006) Cancer Res. 65 (24) :11282-6.;

Other References

Eto K, et al. Loss of programmed cell death 4 induces apoptosis by promoting the translation of procaspase-3 mRNA. (Cell Death Differ. 2012) {https://pubmed.ncbi.nlm.nih.gov/21959934/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl

Scientific Category

Cancer

NCBI Accession Number

NP_055271

Uniprot Accession Number

Q53EL6

Species

Human

AA Sequence

MDVENEQILN VNPADPDNLS DSLFSGDEEN AGTEEIKNEI NGNWISAYSI NEARINAKAK RRLRKNSSRD SGRGDSVSDS GSDALRSGLT VPTSPKGRLL DRRSRSGKGR GLPKKGGAGG KGVWGTPGQV YDVEEVDVKD PNYDDDQENC VYETVVLPLD ERAFEKTLTP IIQEYFEHGD TNEVAEMLRD LNLGEMKSGV PVLAVSLALE GKASHREMTS KLLSDLCGTV MSTTDVEKSF DKLLKDLPEL ALDTPRAPQL VGQFIARAVG DGILCNTYID SYKGTVDCVQ ARAALDKATV LLSMSKGGKR KDSVWGSGGG QQSVNHLVKE IDMLLKEYLL SGDISEAEHC LKELEVPHFH HELVYEAIIM VLESTGESTF KMILDLLKSL WKSSTITVDQ MKRGYERIYN EIPDINLDVP HSYSVLERFV EECFQAGIIS KQLRDLCPSR GRKRFVSEGD GGRLKPESY