Recombinant human Casein kinase 2 alpha 1/CSNK2A1 protein

Protein Kinase Casein Kinase 2 (PKCK2, also called CK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. PKCK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. Recombinant human PKCK2, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Casein kinase 2 alpha 1, Casein kinase II subunit alpha, CK II alpha, CK2A1, Cka1, Cka2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

47.3 kDa (411aa) confirmed by MALDI-TOF

Additionnal Information

Casein Kinase2 a, Casein kinase II alpha 1 subunit isoform a, CSNK2A1, CK2A1, Protein kinase Casein Kinase 2 alpha, PKCK2, CK II, EC 2.7.11.1, Casein kinase II subunit alpha, Casein kinase II alpha 1 subunit isoform a Casein kinase 2 alpha 1 polypeptide, Casein kinase II alpha 1 subunit, Casein kinase II alpha subunit, CK II, CK2 alpha, CK2 catalytic subunit alpha, CK2A1, CKII., PCK0701-10 µg, PCK0701-20 µg, PCK0701-50 µg, PCK0701-100 µg, PCK0701-250 µg, PCK0701-500 µg, PCK0701-1 mg, PCK0701-10, PCK0701-20, PCK0701-50, PCK0701-100, PCK0701-250, PCK0701-500, PCK0701-1

References & Citations

Kim YK., et al. (2006) J Biol Chem. 281 (48) :36752-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 500 mM NaCl, 1mM DTT, 50% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001886

Uniprot Accession Number

P68400

Species

Human

AA Sequence

MSGPVPSRAR VYTDVNTHRP REYWDYESHV VEWGNQDDYQ LVRKLGRGKY SEVFEAINIT NNEKVVVKIL KPVKKKKIKR EIKILENLRG GPNIITLADI VKDPVSRTPA LVFEHVNNTD FKQLYQTLTD YDIRFYMYEI LKALDYCHSM GIMHRDVKPH NVMIDHEHRK LRLIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDYQMYD YSLDMWSLGC MLASMIFRKE PFFHGHDNYD QLVRIAKVLG TEDLYDYIDK YNIELDPRFN DILGRHSRKR WERFVHSENQ HLVSPEALDF LDKLLRYDHQ SRLTAREAME HPYFYTVVKD QARMGSSSMP GGSTPVSSAN MMSGISSVPT PSPLGPLAGS PVIAAANPLG MPVPAAAGAQ Q