Recombinant human PAK4 protein

PAK4 is a member of the group B family of p21-activated kinases (PAK) . It's identified as an effector protein for cell division cycle 42 (Cdc42) and protein that play an important role in regulating cytoskeletal organization and cell morphology. Its expression is elevated in many cancer cell lines, and suggested that it plays an important role in tumorigenesis. Recombinant PAK4 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

P21-activated kinase 4 isoform 1, p21 protein (Cdc42/Rac) -activated kinase 4, Serine/threonine-protein kinase, PAK 4, p21 activated kinase 4 isoform 1, p21-activated kinase 4 isoform 1 KIAA1142, p21 activated kinase 4, p21 (CDKN1A) activated kinase 4, Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs, Serine threonine kinase PAK 4, Serine/threonine protein kinase PAK 4, Serine/threonine protein kinase PAK4.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS-PAGE

Molecular Weight

68.3 kDa (628aa)

Additionnal Information

PAK4, p21-activated kinase 4 isoform 1, p21 protein (Cdc42/Rac) -activated kinase 4, Serine/threonine-protein kinase, PAK 4, p21 activated kinase 4 isoform 1, p21-activated kinase 4 isoform 1 KIAA1142, p21 activated kinase 4, p21 (CDKN1A) activated kinase 4, Protein kinase related to S.cerevisiae STE20 effector for Cdc42Hs, Serine threonine kinase PAK 4, Serine/threonine protein kinase PAK 4, Serine/threonine protein kinase PAK4., PAK0901-10 µg, PAK0901-20 µg, PAK0901-50 µg, PAK0901-100 µg, PAK0901-250 µg, PAK0901-500 µg, PAK0901-1 mg, PAK0901-10, PAK0901-20, PAK0901-50, PAK0901-100, PAK0901-250, PAK0901-500, PAK0901-1

References & Citations

Liu Y., et al. (2008) . Mol Cancer Res. 6 (7) :1215-24.; ; Li X., et al. (2005) . J Biol Chem. 280 (50) :41192-200.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 25mM HEPES buffer (pH 7.4) containing 150 mM NaCl, 5mM MgCl2, 30% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_001014831

Uniprot Accession Number

O96013

Species

Human

AA Sequence

MFG KRKKRVEISA PSNFEHRVHT GFDQHEQKFT GLPRQWQSLI EESARRPKPL VDPACITSIQ PGAPKTIVRG SKGAKDGALT LLLDEFENMS VTRSNSLRRD SPPPPARARQ ENGMPEEPAT TARGGPGKAG SRGRFAGHSE AGGGSGDRRR AGPEKRPKSS REGSGGPQES SRDKRPLSGP DVGTPQPAGL ASGAKLAAGR PFNTYPRADT DHPSRGAQGE PHDVAPNGPS AGGLAIPQSS SSSSRPPTRA RGAPSPGVLG PHASEPQLAP PACTPAAPAV PGPPGPRSPQ REPQRVSHEQ FRAALQLVVD PGDPRSYLDN FIKIGEGSTG IVCIATVRSS GKLVAVKKMD LRKQQRRELL FNEVVIMRDY QHENVVEMYN SYLVGDELWV VMEFLEGGAL TDIVTHTRMN EEQIAAVCLA VLQALSVLHA QGVIHRDIKS DSILLTHDGR VKLSDFGFCA QVSKEVPRRK SLVGTPYWMA PELISRLPYG PEVDIWSLGI MVIEMVDGEP PYFNEPPLKA MKMIRDNLPP RLKNLHKVSP SLKGFLDRLL VRDPAQRATA AELLKHPFLA KAGPPASIVP LMRQNRTR