Recombinant human Serpin E1/PAI-1 protein
Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.
Product Name Alternative
Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Bioactivity
The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.
Molecular Weight
45 kDa (400aa)
Additionnal Information
References & Citations
Gorlatova NV., et al. (2007) J Biol Chem. 282 (12) :9288-96.; ; Renckens R., et al. (2006) J Immunol. 177 (11) :8171-6.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 50 mM NaAc (pH 5.5) containing 10% glycerol, 0.1M NaCl
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_000593
Uniprot Accession Number
P05121
Species
Human
AA Sequence
Frequently Asked Questions
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