Recombinant human Serpin E1/PAI-1 protein

Plasminogen activator inhibitor-1 (PAI-1), a member of the serine protease inhibitor (serpin) super family. PAI-1 is the principal inhibitor of tissue plasminogen activator and urokinase, the activators of plasminogen and hence fibrinolysis. PAI-1 is mainly produced by the endothelium but is also secreted by other tissue types, such as adipose tissue. Recombinant human PAI-1, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

Product Name Alternative

Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Bioactivity

The IC50 for this effect is less than 3 nM, Measured by its ability to inhibit uPA cleavage of the substrate Z-GGR-AMC.

Molecular Weight

45 kDa (400aa)

Additionnal Information

Serpin E1, PAI1, Plasminogen activator inhibitor-1, PLANH1, SERPINE1, PAI1, PLANH1, Plasminogen activator inhibitor-1, Plasminogen activator inhibitor-1, PAI0701-10 µg, PAI0701-20 µg, PAI0701-50 µg, PAI0701-100 µg, PAI0701-250 µg, PAI0701-500 µg, PAI0701-1 mg, PAI0701-10, PAI0701-20, PAI0701-50, PAI0701-100, PAI0701-250, PAI0701-500, PAI0701-1

References & Citations

Gorlatova NV., et al. (2007) J Biol Chem. 282 (12) :9288-96.; ; Renckens R., et al. (2006) J Immunol. 177 (11) :8171-6.;

Other References

Cho KS, et al. Valproic acid induces astrocyte-dependent neurite outgrowth from cultured rat primary cortical neuron via modulation of tPA/PAI-1 activity. (Glia. 2013) {https://pubmed.ncbi.nlm.nih.gov/23378038/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 50 mM NaAc (pH 5.5) containing 10% glycerol, 0.1M NaCl

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_000593

Uniprot Accession Number

P05121

Species

Human

AA Sequence

MGSSHHHHHH SSGLVPRGSH MVHHPPSYVA HLASDFGVRV FQQVAQASKD RNVVFSPYGV ASVLAMLQLT TGGETQQQIQ AAMGFKIDDK GMAPALRHLY KELMGPWNKD EISTTDAIFV QRDLKLVQGF MPHFFRLFRS TVKQVDFSEV ERARFIINDW VKTHTKGMIS NLLGKGAVDQ LTRLVLVNAL YFNGQWKTPF PDSSTHRRLF HKSDGSTVSV PMMAQTNKFN YTEFTTPDGH YYDILELPYH GDTLSMFIAA PYEKEVPLSA LTNILSAQLI SHWKGNMTRL PRLLVLPKFS LETEVDLRKP LENLGMTDMF RQFQADFTSL SDQEPLHVAQ ALQKVKIEVN ESGTVASSST AVIVSARMAP EEIIMDRPFL FVVRHNPTGT VLFMGQVMEP