Recombinant human OSTF1 protein

Osteoclast-stimulating factor-1 (OSTF1) is an intracellular protein produced by osteoclasts that indirectly induces osteoclast (OCL) formation and bone resorption. OSTF1 is highly expressed in OCL and contains structural domains such as SH3 and ankyrin repeats, which are potentially involved in protein-protein interactions. OSTF1 is ubiquitously expressed analogous to other regulators of OCL activity such as c-Src or transforming growth factor beta. Recombinant human OSTF1 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

25.1 kDa (225aa) confirmed by MALDI-TOF

Additionnal Information

OSTF1, Osteoclast stimulating factor 1, SH3P2, OSF, Osteoclast stimulating factor 1, OSF0905-10 µg, OSF0905-20 µg, OSF0905-50 µg, OSF0905-100 µg, OSF0905-250 µg, OSF0905-500 µg, OSF0905-1 mg, OSF0905-10, OSF0905-20, OSF0905-50, OSF0905-100, OSF0905-250, OSF0905-500, OSF0905-1

References & Citations

Kurihara N., et al. (2001) J Bio Chem. 276 (44) :41035-9; ; Reddy S., et al. (1998) Cell Physiol. 177 (4) :636-45.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

AAH07459

Uniprot Accession Number

Q92882

Species

Human

AA Sequence

MSKPPPKPVK PGEGGQVKVF RALYTFEPRT PDELYFEEGD IIYITDMSDT NWWKGTSKGR TGLIPSNYVA EQAESIDNPL HEAAKRGNLS WLRECLDNRV GVNGLDKAGS TALYWACHGG HKDIVEMLFT QPNIELNQQN KLGDTALHAA AWKGYADIVQ LFLAKGARTD LRNIEKKLAF DMATNAACAS LLKKKQGTDA VRTLSNAEDY LDDEDSDLEH HHHHH