Recombinant human OPG/TNFRSF11B protein

Osteoprotegerin (OPG) is a member of the tumor necrosis factor (TNF) -related family, is referred to as TNFRSF11B and part of the OPG/receptor activator of NF-kB ligand (RANKL) /receptor activator of NF-kB (RANK) triad. This cytokine that lacks any apparent cell-association motifs and exists as a soluble secreted protein, network regulates the differentiation and activation of osteoclasts and hence the critical balance between bone formation (osteoblasts) and bone resorption (osteoclasts) . Recombinant human osteoprotegerin protein, fused to His-tag at C-terminus, was expressed in Hi-5 cell using baculovirus expression system and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Human OPG/TNFRSF11B inhibits Human TRAIL (CAT# ATGP3588) cytotoxicity in the Jurkat human acute T cell leukemia cells. The ED50 range ≤ 8 ng/mL.

Product Name Alternative

Tumor necrosis factor receptor superfamily member 11B, Osteoclastogenesis inhibitory factor, Osteoprotegerin, OCIF, OPG, TR1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells in the presence of 2 ng/mL of human TRAIL (CAT# ATGP3588) . The ED50 range ≤ 8 ng/mL.

Molecular Weight

44.7 kDa (389aa) ; ; (On SDS-PAGE under denatured condition, apparent Molecular weight of glycosylated rhOPG protein will appear at approximately 55 kDa)

Additionnal Information

Osteoprotegerin, TNFRSF11B, Tumor necrosis factor receptor superfamily member 11b, Osteoclastogenesis inhibitory factor, OPG, OCIF, Tumor necrosis factor receptor superfamily, member 11b MGC29565, TNFRSF 11B, TNFRSF11B, TR 1, TR1, Tumor Necrosis Factor Receptor Superfamily Member 11b., OPG0905-10 µg, OPG0905-20 µg, OPG0905-50 µg, OPG0905-100 µg, OPG0905-250 µg, OPG0905-500 µg, OPG0905-1 mg, OPG0905-10, OPG0905-20, OPG0905-50, OPG0905-100, OPG0905-250, OPG0905-500, OPG0905-1

References & Citations

Lacey, D.L. et al. (1998) Cell 93:165-76; ; Yasuda H. et al. (1998) PNAS 95:3597-602 ; ; A. Van Campenhout, Golledge J. (2009) Atherosclerosis 204:321-29;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_002537.3

Uniprot Accession Number

O00300

Species

Human

AA Sequence

ETFPPKY LHYDEETSHQ LLCDKCPPGT YLKQHCTAKW KTVCAPCPDH YYTDSWHTSD ECLYCSPVCK ELQYVKQECN RTHNRVCECK EGRYLEIEFC LKHRSCPPGF GVVQAGTPER NTVCKRCPDG FFSNETSSKA PCRKHTNCSV FGLLLTQKGN ATHDNICSGN SESTQKCGID VTLCEEAFFR FAVPTKFTPN WLSVLVDNLP GTKVNAESVE RIKRQHSSQE QTFQLLKLWK HQNKDQDIVK KIIQDIDLCE NSVQRHIGHA NLTFEQLRSL MESLPGKKVG AEDIEKTIKA CKPSDQILKL LSLWRIKNGD QDTLKGLMHA LKHSKTYHFP KTVTQSLKKT IRFLHSFTMY KLYQKLFLEM IGNQVQSVKI SCL