Recombinant E.coli NusA protein

NusA is a key component in both Prevention and enhancement of transcriptional termination. It is important in both Rho-dependent and intrinsic termination, as well as in lambda and other phage antitermination systems. The gene was first identified by isolation of the nusAl mutation, which restricts bacteriophage l growth by preventing the antitermination activity of the l N protein. NusA is involved in transcriptional antitermination in the cell. It has been shown to specifically aid in read-through of the RNA polymerase genes rpoB and rpoC, as well as in successful synthesis of the ribosomal RNA genes. Recombinant NusA was expressed in E. coli and purified by using conventional chromatography techniques

Product Specifications

Product Name Alternative

Transcription termination/antitermination L factor, ECK3158, JW3158, Transcription termination/antitermination L factor, NusA, Transcription termination/antitermination L factor

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

54 kDa (495aa) confirmed by MALDI-TOF

Additionnal Information

NusA, NUS0801-10 µg, NUS0801-20 µg, NUS0801-50 µg, NUS0801-100 µg, NUS0801-250 µg, NUS0801-500 µg, NUS0801-1 mg, NUS0801-10, NUS0801-20, NUS0801-50, NUS0801-100, NUS0801-250, NUS0801-500, NUS0801-1

References & Citations

Carlomagno MS., et al. (2003) Gene. 308:115-28.; ; Gusarov I., . (2001) Cell. 107 (4) :437-49.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

AAC76203

Uniprot Accession Number

P0AFF6

Species

E.coli

AA Sequence

MNKEILAVVE AVSNEKALPR EKIFEALESA LATATKKKYE QEIDVRVQID RKSGDFDTFR RWLVVDEVTQ PTKEITLEAA RYEDESLNLG DYVEDQIESV TFDRITTQTA KQVIVQKVRE AERAMVVDQF REHEGEIITG VVKKVNRDNI SLDLGNNAEA VILREDMLPR ENFRPGDRVR GVLYSVRPEA RGAQLFVTRS KPEMLIELFR IEVPEIGEEV IEIKAAARDP GSRAKIAVKT NDKRIDPVGA CVGMRGARVQ AVSTELGGER IDIVLWDDNP AQFVINAMAP ADVASIVVDE DKHTMDIAVE AGNLAQAIGR NGQNVRLASQ LSGWELNVMT VDDLQAKHQA EAHAAIDTFT KYLDIDEDFA TVLVEEGFST LEELAYVPMK ELLEIEGLDE PTVEALRERA KNALATIAQA QEESLGDNKP ADDLLNLEGV DRDLAFKLAA RGVCTLEDLA EQGIDDLADI EGLTDEKAGA LIMAARNICW FGDEA