Recombinant human MYL2 protein

Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Slow cardiac myosin regulatory light chain 2, MLC2, CMH10, DKFZp779C0562, Slow cardiac myosin regulatory light chain 2, MYL2, Slow cardiac myosin regulatory light chain 2 Cardiac myosin light chain-2, MLC 2v, MYL 2, Cardiac ventricular myosin light chain 2, RLC of myosin, Myosin light chain 2 regulatory cardiac slow, Myosin light polypeptide 2 regulatory cardiac slow, Myosin regulatory light chain 2 ventricular cardiac muscle isoform, Myosin regulatory light chain 2 ventricular/cardiac muscle isoform, Regulatory light chain of myosin

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

20.9 kDa (186aa) confirmed by MALDI-TOF

Additionnal Information

MYL2, MYL0901-10 µg, MYL0901-20 µg, MYL0901-50 µg, MYL0901-100 µg, MYL0901-250 µg, MYL0901-500 µg, MYL0901-1 mg, MYL0901-10, MYL0901-20, MYL0901-50, MYL0901-100, MYL0901-250, MYL0901-500, MYL0901-1

References & Citations

Macera MJ., et al. (1992) Genomics. 13 (3) :829-31; ; Poetter K., et al. (1996) Nat Genet. 13 (1) :63-9

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM CaCl2

Scientific Category

Actin & Myosin Related

NCBI Accession Number

NP_000423

Uniprot Accession Number

P10916

Species

Human

AA Sequence

MAPKKAKKRA GGANSNVFSM FEQTQIQEFK EAFTIMDQNR DGFIDKNDLR DTFAALGRVN VKNEEIDEMI KEAPGPINFT VFLTMFGEKL KGADPEETIL NAFKVFDPEG KGVLKADYVR EMLTTQAERF SKEEVDQMFA AFPPDVTGNL DYKNLVHIIT HGEEKD