Recombinant human MYL2 protein
Myosin, light chain 2 (MYL2) encodes the regulatory light chain associated with cardiac myosin beta heavy chain. It is an important protein involved in the regulation of myosin ATPase activity in smooth muscle and Ca+ triggers the phosphorylation of regulatory light chain that in turn triggers contraction. Mutations in MYL2 are associated with mid-left ventricular chamber type hypertrophic cardiomyopathy. Recombinant human MYL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Molecular Weight
20.9 kDa (186aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Macera MJ., et al. (1992) Genomics. 13 (3) :829-31; ; Poetter K., et al. (1996) Nat Genet. 13 (1) :63-9
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 5mM CaCl2
Scientific Category
Actin & Myosin Related
NCBI Accession Number
NP_000423
Uniprot Accession Number
P10916
Species
Human
AA Sequence
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