What is the catalog number for Recombinant human GM-CSF protein?

The catalog number for Recombinant human GM-CSF protein is MSF0501-010.

What sizes are available for Recombinant human GM-CSF protein?

Recombinant human GM-CSF protein is available in the following size(s): 10 µg.

Is Recombinant human GM-CSF protein in stock?

Recombinant human GM-CSF protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human GM-CSF protein

Human GM-CSF is a 14. 6 kDa globular protein consisting of 128 amino acid containing two dislufied bonds. GM-CSF is a hematopoietic growth factor that stimulates the development of neutrophils and macrophages and promotes the proliferation and development of early erythroid megakaryocytic and eosinophilic progenitor cells. It is produced in by endothelial cells, monocytes, fibroblasts and T-lymphocytes. GM-CSF inhibits neutrophil migration and enhances the functional activity of the mature end-cells. Recombinant human GM-CSF was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human GM-CSF in a cell proliferation assay using TF-1 human erythroleukemic cell. The ED50 range ≤ 50pg/ml.

Product Name Alternative

Granulocyte-macrophage colony stimulating factor, CSF2, GMCSF, GM-CSF, GM-CSF, Colony-stimulating factor, CSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity, CMCSF, Colony stimulating factor, Colony Stimulating Factor 2, CSF 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, MGC131935, MGC138897, Pluripoietin Alpha

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 50pg/ml.

Molecular Weight

14.6 kDa (128aa) confirmed by MALDI-TOF

Additionnal Information

GM-CSF, Granulocyte-macrophage colony stimulating factor, CSF2, GMCSF, GM-CSF, GM-CSF, Colony-stimulating factor, CSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Granulocyte-macrophage colony stimulating factor Burst Promoting Activity, CMCSF, Colony stimulating factor, Colony Stimulating Factor 2, CSF 2, CSF Alpha, Eosinophil Colony Stimulating Factor, Granulocyte Macrophage Colony Stimulating Factor, MGC131935, MGC138897, Pluripoietin Alpha, MSF0501-10 µg, MSF0501-20 µg, MSF0501-50 µg, MSF0501-100 µg, MSF0501-250 µg, MSF0501-500 µg, MSF0501-1 mg, MSF0501-10, MSF0501-20, MSF0501-50, MSF0501-100, MSF0501-250, MSF0501-500, MSF0501-1

References & Citations

Trapnell BC, et al. (2002) Annu Rev Physiol. 64, 775-80; ; Wong GG, et al (1985) Science, 228, 810-815;

Other References

Poudel B, et al. Collagen I enhances functional activities of human monocyte-derived dendritic cells via discoidin domain receptor 2. (Cell Immunol. 2012) {https://pubmed.ncbi.nlm.nih.gov/23121981/}; ; Kusakabe Y, et al. A standardized blood test for the routine clinical diagnosis of impaired GM-CSF signaling using flow cytometry. (J Immunol Methods. 2014) {https://www.ncbi.nlm.nih.gov/pubmed/25068538}