Recombinant human MGMT protein

O-6-methylguanine-DNA methyltransferase (MGMT) is an enzyme that repairs O-6-methylguanine, a mutagenic DNA base damaged by endogenous and environmental alkylating agents and is involved in the cellular defense against the biological effects of O-6-methylguanine in DNA. MGMT repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. There are few reports that abnormal MGMT expression correlates with the prognosis in human solid cancers. Recombinant MGMT, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.8 kDa (227aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Additionnal Information

MGMT, O-6-methylguanine-DNA methyltransferase, MGMT, EC 2.1.1.63, O-6-methylguanine-DNA-alkyltransferase, O-6-methylguanine-DNA methyltransferase 6 O methylguanine DNA methyltransferase, Agat, AGT, AI267024, MGC107020, Methylated DNA protein cysteine methyltransferase, Methylguanine DNA methyltransferase, O 6 methylguanine DNA alkyltransferase., MMT0901-10 µg, MMT0901-20 µg, MMT0901-50 µg, MMT0901-100 µg, MMT0901-250 µg, MMT0901-500 µg, MMT0901-1 mg, MMT0901-10, MMT0901-20, MMT0901-50, MMT0901-100, MMT0901-250, MMT0901-500, MMT0901-1

References & Citations

Matsukura S., et al. (2001) Annals of Surgical Oncology, 8 (10) :807--816; ; Esteller M., et al. (2002) J Natl Cancer Inst. 2, 26-32.;

Other References

Hongo A, et al. A radioisotope-nondependent high-sensitivity method for measuring the activity of glioblastoma-related O (6) -methylguanine DNA methyltransferase. (Anal Biochem. 2015) {https://pubmed.ncbi.nlm.nih.gov/25173514/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 1mM DTT, 10% glycerol

Scientific Category

Enzymes & Proteases

NCBI Accession Number

NP_002403.3

Uniprot Accession Number

P16455

Species

Human

AA Sequence

MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM QCTAWLNAYF HQPEAIEEFP VPAFHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG LGGSSGLAGA WLKGAGATSG SPPAGRN