Recombinant human MIF protein

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF was expressed in E. coli and purified by conventional chromatography techniques

Product Specifications

Product Name Alternative

Macrophage migration inhibitory factor, GLIF, MMIF, MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor, macrophage migration inhibitory factor (glycosylation-inhibiting factor)

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 98% by SDS-PAGE

Molecular Weight

12 kDa (115aa) confirmed by MALDI-TOF

Additionnal Information

MIF, MIF0801-10 µg, MIF0801-20 µg, MIF0801-50 µg, MIF0801-100 µg, MIF0801-250 µg, MIF0801-500 µg, MIF0801-1 mg, MIF0801-10, MIF0801-20, MIF0801-50, MIF0801-100, MIF0801-250, MIF0801-500, MIF0801-1

References & Citations

Weiser WY., et al. (1989) Proc Natl Acad Sci. 86: 7522-26.; ; Bernhagen J., et al. (1994) Biochemistry. 33: 14144-55.; ; Bucala R., et al. (1996) FASEB J 10: 1607-1613.;