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Recombinant human MIF protein

The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.

Product Specifications

Product Name Alternative

Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

17 kDa (155aa) confirmed by MALDI-TOF

Additionnal Information

MIF, Macrophage migration inhibitory factor, Macrophage migration inhibitory factor, GLIF, MMIF, MIF (1-114aa), MIF, EC 5.3.2.1, Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, Macrophage migration inhibitory factor Glycosylation inhibiting factor, MIF protein, MIF0501-10 µg, MIF0501-20 µg, MIF0501-50 µg, MIF0501-100 µg, MIF0501-250 µg, MIF0501-500 µg, MIF0501-1 mg, MIF0501-10, MIF0501-20, MIF0501-50, MIF0501-100, MIF0501-250, MIF0501-500, MIF0501-1

References & Citations

Weiser WY., et al (1989) Proc Natl Acad Sci. 86: 7522-26.; ; Bernhagen J., et al (1994) Biochemistry. 33: 14144-55.; ; Bucala R., et al. (1996) FASEB J 10: 1607-13.;

Other References

Tsuji S, et al. Effective isolation of RNA aptamer through suppression of PCR bias. (Biochem Biophys Res Commun. 2009) {https://pubmed.ncbi.nlm.nih.gov/19520057/}; ; Tsuji S, et al. RNA aptamer binding to polyhistidine-tag. (Biochem Biophys Res Commun. 2009) {https://pubmed.ncbi.nlm.nih.gov/19520059/}; ; Garai J, et al. Tetralone derivatives are MIF tautomerase inhibitors and attenuate macrophage activation and amplify the hypothermic response in endotoxemic mice. (J Enzyme Inhib Med Chem. 2021) {https://pubmed.ncbi.nlm.nih.gov/34225560/}; ; Garai J, et al. Synthesis and evaluation of a new class of MIF-inhibitors in activated macrophage cells and in experimental septic shock in mice. (Eur J Med Chem. 2023) {https://pubmed.ncbi.nlm.nih.gov/36587420/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_002406.1

Uniprot Accession Number

P14174

Species

Human

AA Sequence

MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA

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