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Recombinant E.coli Maltose Binding Protein/MBP protein

MBP (Maltose Binding Protein) is a protein related with the maltose/maltodextrin system of Escherichia coli, which is responsible for the uptake and efficient catabolism of maltodextrins. It is a complex regulatory and transport system involving many proteins and protein complexes. MBP has been used to increase the yield of its fusion partner in many cases. In addition, MBP is often able to promote the solubility of polypeptides to which it is fused. Recombinant MBP was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Maltose binding protein, malE, E.coli MBP, Escherichia coli MBP, MBP, Maltodextrin-binding protein, MMBP

Expression System

E.coli

Antigen Species

E.coli

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

42 kDa (387aa)

Additionnal Information

Maltose Binding Protein, MBP, Maltose binding protein, E.coli MBP, Escherichia coli MBP, MBP0801-10 µg, MBP0801-20 µg, MBP0801-50 µg, MBP0801-100 µg, MBP0801-250 µg, MBP0801-500 µg, MBP0801-1 mg, MBP0801-10, MBP0801-20, MBP0801-50, MBP0801-100, MBP0801-250, MBP0801-500, MBP0801-1

References & Citations

Fox JD., et al. (2001) . Protein Sci. 10 (3) : 622-30.; ; Riggs P., et al. (2000) . Mol. Biotechnol. 15: 51-63.

Other References

Suderman RJ, et al. Development of polyol-responsive antibody mimetics for single-step protein purification. (Protein Expr Purif. 2017) {https://pubmed.ncbi.nlm.nih.gov/28428153/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0)

Scientific Category

Fusion Tag & Detection

NCBI Accession Number

NP_418458.1

Uniprot Accession Number

P0AEX9

Species

E.coli

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