Recombinant human Livin alpha protein

Livin is a member of the family of inhibitor of apoptosis proteins (IAP) and contains a single copy of a baculovirus IAP repeat (BIR) as well as a RING zinc finger (RZF) domain. Livin has direct interaction with several caspases including caspase-3, -7, and -9. This protein inhibits the activation of caspase-9 induced by Apaf-1, cytochrome c, and dATP. Recombinant human Livin fused to His-tag at N-terminus was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Livin inhibitor of apoptosis isoform alpha, BIRC7, Baculoviral IAP repeat-containing 7, KIAP, MLIAP, RNF50, ML-IAP, KIAP, MLIAP, RNF50, Livin, Livin inhibitor of apoptosis isoform alpha, Livin inhibitor of apoptosis isoform alpha Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, BIRC 7, Kidney inhibitor of apoptosis protein, Livin inhibitor of apotosis, RING finger protein 50, RNF 50, Melanoma inhibitor of apoptosis protein, ML IAP.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

34.9 kDa (318aa) confirmed by MALDI-TOF

Additionnal Information

Livin a, Livin inhibitor of apoptosis isoform alpha, BIRC7, Baculoviral IAP repeat-containing 7, KIAP, MLIAP, RNF50, ML-IAP, KIAP, MLIAP, RNF50, Livin, Livin inhibitor of apoptosis isoform alpha, Livin inhibitor of apoptosis isoform alpha Baculoviral IAP repeat containing 7, Baculoviral IAP repeat containing protein 7, BIRC 7, Kidney inhibitor of apoptosis protein, Livin inhibitor of apotosis, RING finger protein 50, RNF 50, Melanoma inhibitor of apoptosis protein, ML IAP., LIV0801-10 µg, LIV0801-20 µg, LIV0801-50 µg, LIV0801-100 µg, LIV0801-250 µg, LIV0801-500 µg, LIV0801-1 mg, LIV0801-10, LIV0801-20, LIV0801-50, LIV0801-100, LIV0801-250, LIV0801-500, LIV0801-1

References & Citations

Kasof GM., et al. (2001) . J Biol Chem. 276 (5) :3238-46.; ; Ashhab Y., et al. (2001) . FEBS Lett. 495 (1-2) :56-60.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 2mM EDTA, 20% glycerol

Scientific Category

Cancer

NCBI Accession Number

NP_647478

Uniprot Accession Number

Q96CA5

Species

Human

AA Sequence

MGPKDSAKCL HRGPQPSHWA AGDGPTQERC GPRSLGSPVL GLDTCRAWDH VDGQILGQLR PLTEEEEEEG AGATLSRGPA FPGMGSEELR LASFYDWPLT AEVPPELLAA AGFFHTGHQD KVRCFFCYGG LQSWKRGDDP WTEHAKWFPS CQFLLRSKGR DFVHSVQETH SQLLGSWDPW EEPEDAAPVA PSVPASGYPE LPTPRREVQS ESAQEPGGVS PAEAQRAWWV LEPPGARDVE AQLRRLQEER TCKVCLDRAV SIVFVPCGHL VCAECAPGLQ LCPICRAPVR SRVRTFLS