Recombinant mouse Leptin/OB protein

Leptin is a 16-kDa nonglycosylated hormone that is produced in mature adipocytes. Leptin acts primarily in the hypothalamus to reduce food intake and body weight. In ob/ob mice, the gene encoding leptin is mutated, resulting in morbid obesity and associated abnormalities, including hyperphagia, hypothermia, diabetes and infertility. Originally discovered in the mouse, the cDNA for leptin has been so far cloned from a variety of species including human, rat, cow, chicken, pig, sheep and dog. Recombinant mouse leptin was overexpressed as insoluble protein aggregate in E. coli and purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer

Product Specifications

Product Name Alternative

LEP, OB, OBS, Leptin Mouse, Obesity factor, Obese protein, Leptin

Expression System

E.coli

Antigen Species

Mouse

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

16 kDa (147aa) confirmed by MALDI-TOF

Additionnal Information

Leptin, OB, LEP, OB, OBS, Leptin Mouse, Obesity factor, Obese protein, Leptin, LEP0701-10 µg, LEP0701-20 µg, LEP0701-50 µg, LEP0701-100 µg, LEP0701-250 µg, LEP0701-500 µg, LEP0701-1 mg, LEP0701-10, LEP0701-20, LEP0701-50, LEP0701-100, LEP0701-250, LEP0701-500, LEP0701-1

References & Citations

Jeong KJ., et al. (1999) Appl Environ Microbiol. 65 (7), 3027-32.; ; Anini Y., et al. (2003) Diabetes. 52 (2), 252-259.; ; Hyogo H., et al. (2002) J. Biol. Chem. 277 (37), 34117-34124.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032519

Uniprot Accession Number

P41160

Species

Mouse

AA Sequence

MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC