Recombinant human CD158e/KIR3DL1 protein
The three Ig-domain from of inhibitory killer cell Ig-like receptor 1 (KIR3DL1, NKB1, nkat3, p70KIR) is a NK cell receptor for polymorphic HLA-B determinant. KIR3DL1 recognizes the Bw4 determinant defined by sequence motifs at positins 77-83 of the HLA-B heavy chain. The cytoplasmic tail of KIR, which contains two immunoreceptor tyrosine-based inhibition motifs (ITIMs), mediates inhibitory signal transduction that prevents killer cell-mediated cytotoxicity. A His-tag fusion protein of KIR3DL1 cytoplasmic tail (361-444aa) was overexpressed as insoluble protein aggregates (inclusion bodies) . This protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
15 kDa (132aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Chan HW., et al. (2003) J. Exp. Med. 197 (2), 245-55.; ; Gardiner CM., et al . (2001) J Immunol. 166 (5) :2992-3001.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 25mM Tris-HCl buffer (pH 7.5) containing 100 mM NaCl
Scientific Category
Immunology
NCBI Accession Number
NP_037421
Uniprot Accession Number
P43629
Species
Human
AA Sequence
MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSTSGT IDKLDIEFHL WCSNKKNAAV MDQEPAGNRT ANSEDSDEQD PEEVTYAQLD HCVFTQRKIT RPSQRPKTPP TDTILYTELP NAKPRSKVVS CP
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