Recombinant human CD158i/KIR2DS4 protein

An activating Killer Cell Ig-like Receptor (KIR, previously called p50 KIR, p50. 3, cl39, or KAR-K1), which may recognize class I MHC molecules. The protein coding region of the extracellular domain of KIR2DS4 (amino acids 1-202) was cloned into an E. coli expression vector. The extracellular domain of KIR2DS4 was overexpressed as insoluble protein aggregates (inclusion bodies) . The recombinant KIR2DS4 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor two Ig domains and short cytoplasmic tail 4, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen-like family member I, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clones CL-39/CL-17, p58 NK receptor CL-39/CL-17, KKA3, cl-39

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.2 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

KIR2DS4, CD158i, Killer cell immunoglobulin-like receptor 2DS4, KIR2DS4, CD158I, KKA3, NKAT8, KIR2DS4, p50 KIR, CD158, MHC class I NK cell receptor, Natural killer-associated transcript 8, NKAT-8, P58 natural killer cell receptor clone CL-39, p58 NK receptor, CL-17, CD158 antigen-like family member I, CD15, Killer cell immunoglobulin-like receptor 2DS4, Killer cell immunoglobulin-like receptor 2DS4, CD158 antigen like family member I, CD158i antigen, CL 17, CL 39, CL17, CL39, Killer cell immunoglobulin like receptor two domains short cytoplasmic tail 4, Killer inhibitory receptor 4 1 2, KIR antigen 2DS4, KIR1D, KIR412, MGC120019, MGC125315, MGC125317, MHC class I NK cell receptor, Natural killer associated transcript 8, Natural killer cell inhibitory receptor, NKAT 8, P58 natural killer cell receptor clone CL 39, p58 NK receptor, KIR3003-10 µg, KIR3003-20 µg, KIR3003-50 µg, KIR3003-100 µg, KIR3003-250 µg, KIR3003-500 µg, KIR3003-1 mg, KIR3003-10, KIR3003-20, KIR3003-50, KIR3003-100, KIR3003-250, KIR3003-5

References & Citations

Steffens u., et al. (1998) Tissue Antigens 51, 398-413.; ; Wagtmann N., et al. (1995) Immunity. 2, 439-449.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Immunology

NCBI Accession Number

NP_036446

Uniprot Accession Number

P43632

Species

Human

AA Sequence

MEGVHRKPSF LALPGHLVKS EETVILQCWS DVMFEHFLLH REGKFNNTLH LIGEHHDGVS KANFSIGPMM PVLAGTYRCY GSVPHSPYQL SAPSDPLDMV IIGLYEKPSL SAQPGPTVQA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAVRSINGTF QADFPLGPAT HGGTYRCFGS FRDAPYEWSN SSDPLLVSVT GN