Recombinant human CD158i/KIR2DS4 protein
An activating Killer Cell Ig-like Receptor (KIR, previously called p50 KIR, p50. 3, cl39, or KAR-K1), which may recognize class I MHC molecules. The protein coding region of the extracellular domain of KIR2DS4 (amino acids 1-202) was cloned into an E. coli expression vector. The extracellular domain of KIR2DS4 was overexpressed as insoluble protein aggregates (inclusion bodies) . The recombinant KIR2DS4 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
22.2 kDa (202aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Steffens u., et al. (1998) Tissue Antigens 51, 398-413.; ; Wagtmann N., et al. (1995) Immunity. 2, 439-449.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5)
Scientific Category
Immunology
NCBI Accession Number
NP_036446
Uniprot Accession Number
P43632
Species
Human
AA Sequence
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