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Recombinant human CD158b2/KIR2DL3 protein

An inhibitory Killer Cell Ig-like Receptor (KIR, previously called p58 KIR, cl-6, NKAT2 or KIR-K7), which recognizes class I MHC molecules (HLA-Cw1, -Cw3, -Cw7, and Cw8) . The protein coding region of the extracellular domain of KIR2DL3 (22-223aa) was cloned into an E. coli expression vector. The extracellular domain of KIR2DL3 protein was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

Product Specifications

Product Name Alternative

Killer cell immunoglobulin like receptor, Two Ig domains and long cytoplasmic tail 3, Killer cell immunoglobulin-like receptor 2DL3, CD158 antigen-like family member B2, KIR-023GB, Killer inhibitory receptor cl 2-3, NKAT2a, NKAT2b, Natural killer-associated transcript 2, NKAT-2, p58 natural killer cell receptor clone CL-6, p58 NK receptor CL-6, p58.2 MHC class-I-specific NK receptor, CD158B2, KIRCL23, p58

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

22.2 kDa (202aa) confirmed by MALDI-TOF

Additionnal Information

KIR2DL3, CD158b2, Killer cell immunoglobulin-like receptor 2DL3, KIR2DL3, CD158B2, KIRCL23, NKAT2, KIR2DL3 (p58 KIR, CD158), MHC class I NK cell receptor, Natural killer-associated transcript 2, NKAT-2, NKAT2a, NKAT2b, p58 natural killer cell receptor clone CL-6, p58 NK receptor, p58.2 MHC class-I-specific NK re, Killer cell immunoglobulin-like receptor 2DL3, Killer cell immunoglobulin-like receptor 2DL3 CD158b, GL183, Killer Cell Immunoglobulin Like Receptor 2DL3, Killer inhibitory receptor CL2 3, KIR023GB, MHC Class I NK Cell Receptor, Natural Killer Associated Transcript 2, p58, p58 Natural Killer Cell Receptor Clone CL6, p58.2 MHC Class I Specific NK Receptor, KIR3002-10 µg, KIR3002-20 µg, KIR3002-50 µg, KIR3002-100 µg, KIR3002-250 µg, KIR3002-500 µg, KIR3002-1 mg, KIR3002-10, KIR3002-20, KIR3002-50, KIR3002-100, KIR3002-250, KIR3002-500, KIR3002-1

References & Citations

Steffens, u. et al. (1998) Tissue Antigens 51, 398-413.; ; Wagtmann, N. R., et al. (1995) Immunity 2, 439-449.; ; Colonna, M. et al. (1995) Science 268, 405-408.; ; Kim, J. et al. (1997) J. Immunol. 159, 3875-3882;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5)

Scientific Category

Immunology

NCBI Accession Number

NP_056952.2

Uniprot Accession Number

P43628

Species

Human

AA Sequence

MEGVHRKPSL LAHPGPLVKS EETVILQCWS DVRFQHFLLH REGKFKDTLH LIGEHHDGIS KANFSIGPMM QDLAGTYRCY GSVTHSPYQL SAPSDPLDIV ITGLYEKPSL SAQPGPTVLA GESVTLSCSS RSSYDMYHLS REGEAHERRF SAGPKVNGTF QADFPLGPAT HGGTYRCFGS FRDSPYEWSN SSDPLLVSVT GN

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