Recombinant human ISG15 protein

The interferon-stimulated gene, 15 kDa (ISG15) is an interferon regulated gene that is induced as a primary response to diverse microbial and cell stress stimuli, and encodes the founding member of the ubiquitin-like protein family. ISG15 shares several common properties with other ubiquitin-like molecules (uBLs), but its activity is tightly regulated by specific signaling pathways that have a role in innate immunity. Recombinant human ISG15 was expressed in E. coli and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15, ISG15, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDa protein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, Interferon Stimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor.

Expression System

E.coli

Antigen Species

Human

Tag

Calmodulin Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 98% by SDS-PAGE

Molecular Weight

34 kDa (308aa) confirmed by MALDI-TOF

Additionnal Information

ISG15, Interferon-stimulated gene 15 kDa ubiquitin-like modifier, GIP2, uCRP, IFI15, ISG15, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier, Interferon-stimulated gene, 15 kDa ubiquitin-like modifier IFI 15, Interferon alpha inducible protein, Interferon induced 15 kDa protein, Interferon induced 17 kDa protein, Interferon induced 17 kDa/15 kDa protein, Interferon Stimulated Gene 15, Interferon stimulated protein 15 kDa, ISG 15, ISG15 ubiquitin like modifier, ubiquitin cross reactive protein precursor., ISG0801-10 µg, ISG0801-20 µg, ISG0801-50 µg, ISG0801-100 µg, ISG0801-250 µg, ISG0801-500 µg, ISG0801-1 mg, ISG0801-10, ISG0801-20, ISG0801-50, ISG0801-100, ISG0801-250, ISG0801-500, ISG0801-1

References & Citations

Narasimhan J., et al. (2005) J Biol Chem. 280 (29) :27356-65.; ; Andersen JB., et al. (2006) Cytokine Growth Factor Rev. 17 (6) :411-21 ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 1mM DTT

Scientific Category

Ubiquitination

NCBI Accession Number

NP_005092.1

Uniprot Accession Number

P05161

Species

Human

AA Sequence

MGWDLTVKM LAGNEFQVSL SSSMSVSELK AQITQKIGVH AFQQRLAVHP SGVALQDRVP LASQGLGPGS TVLLVVDKCD EPLNILVRNN KGRSSTYEVR LTQTVAHLKQ QVSGLEGVQD DLFWLTFEGK PLEDQLPLGE YGLKPLSTVF MNLRLRGG