Recombinant human IPP-1/PPP1R1A protein
Protein phosphatase inhibitor-1 (IPP-1) plays an important role in the regulation of glycogen metabolism through inhibition of type-1 protein serine/threonine phosphatase (PP1) activity, and it has been implicated in the regulation of cell growth. IPP-1 activation may impose cAMP control over proteins that are not directly phosphorylated by PKA. In the presence of calcium, PPI-1 is inactivated by calcineurin (or PP2B) . Recombinant human IPP-1, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 85% by SDS-PAGE
Molecular Weight
20kDa (179aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Weiser DC., et al. (2004), J. Biol. Chem. 279 (47) :48904-14; ; Rodriguez P., et al. (2006), J. Biol. Chem. 281 (50) :38599-608;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1mM PMSF, 1mM EDTA, 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_006732.3
Uniprot Accession Number
Q13522
Species
Human
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