Recombinant human IL-3 protein
Interleukin-3 (IL-3) stimulates the proliferation of hematopoietic pluripotent progenitor cells. It is secreted by activated T cells to support growth and differentiation of T cells from the bone marrow in an immune response. IL-3 exerts its biological activities through binding to specific cell surface receptors. IL-3 receptor is a complex consisting of alpha and beta subunits, which in turn induces activation of JAK2/ STAT5, induction of c-myc, and activation of the Ras pathway. Human IL3 is a protein of 15-17 kDa (133 amino acids) . It is synthesized as a precursor containing a hydrophobic secretory signal sequence of 19 amino acids. IL3 contains two putative glycosylation sites at positions 15 and 70 and contains a single disulfide bond (Cys16/84) . Recombinant human IL-3 was expressed in E. coli and purified by using conventional chromatography after refolding of the isolated inclusion bodies in a redox buffer.
Product Specifications
Specifications
Human IL-3 stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range is less or equal 0.15 ng/mL.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effects less or equal to 0.15 ng/mL.
Molecular Weight
17.3 kDa (154aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; ; Michaud DS., et al. (2006), Cancer Res. 66 (8) :4525-30;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol, 0.2mM PMSF
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000579
Uniprot Accession Number
P08700
Species
Human
AA Sequence
APMTQTTSL KTSWVNCSNM IDEIITHLKQ PPLPLLDFNN LNGEDQDILM ENNLRRPNLE AFNRAVKSLQ NASAIESILK NLLPCLPLAT AAPTRHPIHI KDGDWNEFRR KLTFYLKTLE NAQAQQTTLS LAIF
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