Recombinant mouse IL-1 beta/IL1B protein

IL-1beta is an inflammatory cytokine that might modulate angiogenesis by directly interacting with vascular endothelial cells or enhancing the production of proangiogenic factors via paracrine control. IL-1beta stimulates endothelial cell migration and proliferation, adhesion-molecule expression, inflammatory mediator production, and leukocyte recruitment. IL-1beta is required for tumor growth, metastasis, and angiogenesis in several animal models. Recombinant mouse IL-1beta, fused to His tag, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-1 beta, IL-1 beta

Expression System

E.coli

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

21 kDa (189aa) confirmed by MALDI-TOF

Additionnal Information

Pro interleukin 1 beta, Preinterleukin beta, Preinterleukin 1 beta, OTTHUMP00000162031, Osteoclast activating factor, OAF, Mouse IL1 beta, LAF, Interleukin-1 beta, Interleukin 1 beta precursor, Interleukin 1 beta Catabolin, Interleukin 1 beta, IL1F2, IL-1beta, IL1B_HUMAN, IL-1b, IL1B, IL1 F2, IL-1 beta, IL1 BETA, IL1 b, IL 1B, IL 1 beta, IL 1, IFN beta inducing factor, Hematopoietin 1, H1, Catabolin, ILB0801-10 µg, ILB0801-20 µg, ILB0801-50 µg, ILB0801-100 µg, ILB0801-250 µg, ILB0801-500 µg, ILB0801-1 mg, ILB0801-010, ILB0801-020, ILB0801-050, ILB0801-100, ILB0801-250, ILB0801-500, ILB0801-01M

References & Citations

Saijo Y, et al, (2002) J. Immunol. 169:469-475.; ; Torisu H, et al, (2000) J. Cancer. 85:182-188.;

Other References

Yang JC et al. Determination of tumor necrosis factor-α in serum using extended-gate field-effect transistor-based chemosensors with molecularly imprinted polymer-coated gold dendrites. (Sens Actuators B Chem. 2023) {https://doi.org/10.1016/j.snb.2023.133982}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing, 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_032387

Uniprot Accession Number

P10749

Species

Mouse

AA Sequence

MVPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS