Recombinant human IL-1 beta/IL1B protein

Interleukin-1 (IL-1) has been considered a potentially important inflammatory mediator. It is composed of two distinct proteins, called as IL-1alpha and IL-1beta. IL-1beta is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1alpha and IL-1beta bind to the same receptor and they are structurally related polypeptides that share approximately 20% amino acid. Recombinant human IL-1beta was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human IL-1 beta /IL-1F2 stimulates cell proliferation of the D10.G4.1 mouse helper T cells. The ED50 range ≤ 12 pg/ml.

Product Name Alternative

Interleukin-1 beta, IL-1 beta, Catabolin, IL1F2

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 range ≤ 12pg/ml.

Molecular Weight

17 kDa (154aa) confirmed by MALDI-TOF

Additionnal Information

IL1 b, IL1F2, ILB0701-10 µg, ILB0701-20 µg, ILB0701-50 µg, ILB0701-100 µg, ILB0701-250 µg, ILB0701-500 µg, ILB0701-1 mg, ILB0701-10, ILB0701-20, ILB0701-50, ILB0701-100, ILB0701-250, ILB0701-500, ILB0701-1

References & Citations

Allan SM., et al. (2005), Nat. Rev. Immunol. 5:629.; ; Michaud DS., et al. (2006), Cancer Res.66 (8) :4525-30;

Other References

Choi DY et al., Molecularly imprinted polymer-based electrochemical impedimetric sensors on screen-printed carbon electrodes for the detection of trace cytokine IL-1β. (Biosens Bioelectron. 2022) {https://pubmed.ncbi.nlm.nih.gov/35144170/}; ; Park RW et al., Mobile Point-of-Care Device Using Molecularly Imprinted Polymer-Based Chemosensors Targeting Interleukin-1β Biomarker. (Biosensors. 2023) {https://pubmed.ncbi.nlm.nih.gov/38131773/}; ; Kim MJ et al., Potential Chondroprotective Effect of Artemisia annua L. Water Extract on SW1353 Cell. (Int. J. Mol. Sci. 2025) {https://pubmed.ncbi.nlm.nih.gov/40076528/}; ; Kim Y et al., Molecularly imprinted polymer-based electrochemical sensor for IL-6 detection with senescence cell models. (Sensors and Actuators B: Chemical. 2025) {https://doi.org/10.1016/j.snb.2025.137805}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000567.1

Uniprot Accession Number

P01584

Species

Human

AA Sequence

MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS