Recombinant human IL-1Ra/IL1RN protein

One of the most potent pro-inflammatory mediators is the early-acting cytokine interleukin-1 (IL-1), whose actions are regulated by the structurally related IL-1 receptor antagonist (IL-1 RA) . IL-1 RA is a competitive IL-1 inhibitor and a powerful anti-inflammatory agent. Also, Several autoimmune and inflammatory diseases have been associated with an allelic polymorphism of the IL-1 RA gene. Recombinant human IL-1 RA was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interleukin-1 receptor antagonist protein, IL-1RN, IL-1ra, IRAP, ICIL-1RA, IL1 inhibitor, IL1F3, IL1RA

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

17.2 kDa (153aa) confirmed by MALDI-TOF

Additionnal Information

IL1ra, IL1F3, IL1RN, IL1F3, IL1RA, Interleukin-1 receptor antagonist, IL-1ra, IL-1RN, IRAP, IL1 inhibitor, ICIL-1RA, Anakinra, Interleukin-1 receptor antagonist protein, ICIL 1RA, IL1 inhibitor, ICIL1RA, ILA0501-10 µg, ILA0501-20 µg, ILA0501-50 µg, ILA0501-100 µg, ILA0501-250 µg, ILA0501-500 µg, ILA0501-1 mg, ILA0501-10, ILA0501-20, ILA0501-50, ILA0501-100, ILA0501-250, ILA0501-500, ILA0501-1

References & Citations

Capper., et al. (2004) Clin. Exp. Immunol. 138:348 ; ; Roberge CJ., et al. (1994) Journal of Immunology. 152: 5485-94 ; ; Carter DB., et al. (1990) Nature. 344: 633-7 ;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_776214

Uniprot Accession Number

P18510

Species

Human

AA Sequence

MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE