Recombinant human IGF-1 protein
The Insulin-like growth factor-1 (IGF-1) is mitogenic polypeptide growth factors that stimulate the proliferation and survival of various cell types including muscle, bone, and cartilage tissue in vitro. IGF-1 is predominantly produced by the liver, although a variety of tissues produce the IGFs at distinctive times. The IGF-1 belongs to the insulin gene family, which also contains insulin and relaxin. The IGF-1 is similar by structure and function to insulin, but have a much higher growth-promoting activity than insulin. This recombinant human IGF-I is globular protein containing 70 amino acids, and 3 intra-molecular disulfide bonds. Recombinant IGF-1 was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Specifications
Specifications
Human IGF-I stimulates cell proliferation of the MCF-7 human breast cancer cells. The ED50 range ≤ 5 ng/mL.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured in a cell proliferation assay using MCF-7 human breast cancer cells. The ED50 range ≤ 5 ng/mL.
Molecular Weight
7.7 kDa (71aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4)
Scientific Category
Hormones & Cytokines
NCBI Accession Number
NP_000609.1
Uniprot Accession Number
P05019
Species
Human
AA Sequence
MGPETLCGAE LVDALQFVCG DRGFYFNKPT GYGSSSRRAP QTGIVDECCF RSCDLRRLEM YCAPLKPAKS A
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