Recombinant human IFN-alpha 2/IFNA2 protein

Interferon induces a variety of effects on target cells including antiviral, antiproliferative, and immunomodulatory activities. Interferon alpha-2b can either inhibit or enhance immune cell activity within the tightly regulated microenvironment of inflammation, depending upon the concentration of the cytokine and the activation stage of the cell. Interferon alpha-2b was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Specifications

Human IFN-alpha 2 is coated at 10 µg/mL (100 ul/well) can bind Human IFN-alpha/beta R2 (CAT# ATGP3952) in the presence of Human IFN-alpha/beta R1 (CAT# ATGP3894) .

Product Name Alternative

Interferon alpha 2, IFN-alpha-2, Interferon alpha-A, IFNA, IFN-alphaA, LeIF A, IFNA2A, IFNA2B, IFNA2C, alpha-2a interferon, Interferon alpha 2b

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human IFN-alpha/beta R2 (CAT# ATGP3952) in the presence of Human IFN-alpha/beta R1 (CAT# ATGP3894) .

Molecular Weight

19.4 kDa (166aa) confirmed by MALDI-TOF

Additionnal Information

IFN-a2, Interferon a-2b, IRF-2-binding protein 1, Interferon regulatory factor 2-binding protein 1, interferon regulatory factor 2 binding protein 1, DKFZP434M154, IFN0502-10 µg, IFN0502-20 µg, IFN0502-50 µg, IFN0502-100 µg, IFN0502-250 µg, IFN0502-500 µg, IFN0502-1 mg, IFN0502-10, IFN0502-20, IFN0502-50, IFN0502-100, IFN0502-250, IFN0502-500, IFN0502-1

References & Citations

Mire-Sluis AR., et al, (1996) J. Immunol. Methods.195, 55-61.; ; Rubinstein S., et al. (1981) J. Virol. 37, 755-758.;

Other References

Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009) {https://pubmed.ncbi.nlm.nih.gov/19259669/} {}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4), 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_000596.2

Uniprot Accession Number

P01563

Species

Human

AA Sequence

MCDLPQTHSL GSRRTLMLLA QMRRISLFSC LKDRHDFGFP QEEFGNQFQK AETIPVLHEM IQQIFNLFST KDSSAAWDET LLDKFYTELY QQLNDLEACV IQGVGVTETP LMKEDSILAV RKYFQRITLY LKEKKYSPCA WEVVRAEIMR SFSLSTNLQE SLRSKE