Recombinant human IFN-alpha 1/IFNA1 protein

Interferon- alpha is cytokines that are widely known to induce potent anti-viral activity. Interferon -alpha exerts a variety of other biological effects, including antitumor and immunomodulatory activities and are increasingly used clinically to treat a range of malignancies, myelodysplasias and autoimmune diseases. Recombinant human Interferon-alpha was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Specifications

Product Name Alternative

Interferon alpha 1, IFN-alpha-1/13, Interferon alpha-D, LeIF D, IFN-alpha 1b, Interferon alpha 1b, IFNA@, IFL, IFN, IFN-ALPHA, IFNA13, IFN-alphaD

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

19.5 kDa (167aa) confirmed by MALDI-TOF

Additionnal Information

Interferon a1, Ifna1, Interferon a 1, Interferon alpha-1, IFN-ALPHA, IFL, IFNA13, IFN, IFN alpha, IFNA, IFNA1, Interferon alpha 1, Interferon alpha 1/13, Interferon alpha 13, Interferon alpha D, Interferon alpha leukocyte, Interferon leukocyte, LeIF D, MGC138207, MGC138505, MGC138507, IFN0501-10 µg, IFN0501-20 µg, IFN0501-50 µg, IFN0501-100 µg, IFN0501-250 µg, IFN0501-500 µg, IFN0501-1 mg, IFN0501-10, IFN0501-20, IFN0501-50, IFN0501-100, IFN0501-250, IFN0501-500, IFN0501-1

References & Citations

Goeddel DV, et al. (1981) Nature, 290 (5801) : 20-6 ; ; Mire-Sluis, A.R., et al. (1996) J. Immunol. Methods.195: 55-61; ; Rubinstein, S., et al. (1981) J. Virol. 37: 755-758;

Other References

Kim YM, et al. Expression of human interferon alpha-1 with enhanced stability via the tagging system of a stabilizing peptide. (Protein Expr Purif. 2009) {https://pubmed.ncbi.nlm.nih.gov/18950714/}; ; Kim HR, et al. Anti-cancer activity and mechanistic features of a NK cell activating molecule. (Cancer Immunol Immunother. 2009) {https://pubmed.ncbi.nlm.nih.gov/19259669/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_076918.1

Uniprot Accession Number

P01562

Species

Human

AA Sequence

MCDLPETHSL DNRRTLMLLA QMSRISPSSC LMDRHDFGFP QEEFDGNQFQ KAPAISVLHE LIQQIFNLFT TKDSSAAWDE DLLDKFCTEL YQQLNDLEAC VMQEERVGET PLMNADSILA VKKYFRRITL YLTEKKYSPC AWEVVRAEIM RSLSLSTNLQ ERLRRKE