Recombinant human Hexokinase 2 protein
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase2 is found in the skeletal muscle and includes hydrophobic N-terminal sequence capable of targeting the hexokinase to mitochondria. Recombinant human Hexokinase2, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 12,000 pmol/min/ug. One unit will convert 1 pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
Product Name Alternative
HK2, Hexokinase 2, EC 2.7.1.1, Hexokinase type II, HK II, Muscle form hexokinase, Hexokinase-2, hexokinase 2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 85% by SDS-PAGE
Bioactivity
Specific activity is > 12,000pmol/min/ug. One unit will convert 1pmole of D-Glucose to D-Glucose-6-phosphate per minute at pH8.0 at 37C.
Molecular Weight
104.1 kDa (937aa)
Additionnal Information
References & Citations
Jon E. et al., (2003) J.Exp Biology. 206 : 2049-2057.; ; Furuta H. et al., (1996) Genomics. 36 (1) :206-9.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_000180
Uniprot Accession Number
P52789
Species
Human
AA Sequence
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