Recombinant human Hexokinase 3 protein
Hexokinase is the first enzyme in the glycolytic pathway, catalyzing the transfer of a phosphoryl group from ATP to glucose to form glucose-6-phosphate and ADP. In mammals, four distinct enzymes-types 1 to 4 hexokinases-have been identified. The enzyme is found in most cells, but there is tissue specificity for the particular type of hexokinase. Hexokinase3 lacks the hydrophobic N-terminal sequence critical for targeting to mitochondria. Hexpkinase3 may have anabolic functions, providing H6P for glycogen or lipid synthesis. Recombinant human Hexokinase3, fused to His tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
HK3, Hexokinase 3, EC 2.7.1.1, Hexokinase type III, HK III, Hexokinase-3, HKIII, Hexokinase3, HK3, HXK3.
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
101.1 kDa (943aa)
Additionnal Information
References & Citations
Jon E. et al., (2003) J.Exp Biology. 206 : 2049-2057; ; Furuta H. et al., (1996) Genomics. 1996; ; 36 (1) :206-9.;
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002106.2
Uniprot Accession Number
P52790
Species
Human
AA Sequence
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