Recombinant human HSP47/SERPINH1 protein

Description: Heat shock protein 47 (HSP47), also known as Colligin or Serpin H1, is a serpin which serves as a human chaperone protein for collagen. This protein is a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein acts as a molecular chaperone facilitating the folding and assembly of procollagen molecules, retaining unfolded molecules within the ER, and assisting the transport of correctly folded-molecules from the ER to Golgi apparatus. Recombinant human HSP47/Colligin protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Serpin H1, Serpin family H member 1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3, Cell proliferation-inducing gene 14 protein, Collagen-binding protein, Colligin, Collagen binding protein 1, Rheumatoid arthritis-related antigen RA-A47CBP1, CBP2, SERPINH2, PIG14, Heat shock protein 47

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Molecular Weight

48.9 kDa (439aa) confirmed by MALDI-TOF

Additionnal Information

HSP47, Colligin, Collagen-binding protein, Serpin H1, SERPINH1, RA-A47, AsTP3, Heme oxygenase 1, HSP47, Collogin, 47 kDa heat shock protein, 47 kDa heat shock protein precursor, Arsenic transactivated protein 3, AsTP 3, CBP 1, CBP 2, CBP1, CBP2, Collagen binding protein, SERPINH2, Collagen binding protein 1, Collagen binding protein 2, Colligen, Colligin 1, Colligin 2, gp46, Heat shock protein 47, HGNC 1547, Hsp 47, J6, PIG 14, PIG14, PPROM, Proliferation inducing gene 14, Proliferation inducing gene 14 protein, RA A47, Rheumatoid arthritis antigen A 47, Rheumatoid arthritis related antigen RA A47, Serine or cysteine proteinase inhibitor clade H member 1, Serine or cysteine proteinase inhibitor clade H member 2, Serpin H1, Serpin peptidase inhibitor clade H member 1., HSP0904-10 µg, HSP0904-20 µg, HSP0904-50 µg, HSP0904-100 µg, HSP0904-250 µg, HSP0904-500 µg, HSP0904-1 mg, HSP0904-10, HSP0904-20, HSP0904-50, HSP0904-100, HSP0904-250, HSP0904-500, HSP0904-1

References & Citations

Mohammed Tasab., et al. (2000) The EMBO Journal. 19 (10) : 2204-2211; ; Maloney A., et al. (2007) Cancer Res. 67 (7) :3239-53;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_001226.2

Uniprot Accession Number

P50454

Species

Human

AA Sequence

AA EVKKPAAAAA PGTAEKLSPK AATLAERSAG LAFSLYQAMA KDQAVENILV SPVVVASSLG LVSLGGKATT ASQAKAVLSA EQLRDEEVHA GLGELLRSLS NSTARNVTWK LGSRLYGPSS VSFADDFVRS SKQHYNCEHS KINFRDKRSA LQSINEWAAQ TTDGKLPEVT KDVERTDGAL LVNAMFFKPH WDEKFHHKMV DNRGFMVTRS YTVGVMMMHR TGLYNYYDDE KEKLQIVEMP LAHKLSSLII LMPHHVEPLE RLEKLLTKEQ LKIWMGKMQK KAVAISLPKG VVEVTHDLQK HLAGLGLTEA IDKNKADLSR MSGKKDLYLA SVFHATAFEL DTDGNPFDQD IYGREELRSP KLFYADHPFI FLVRDTQSGS LLFIGRLVRP KGDKMRDEL