Recombinant human GRP75/HSPA9B protein

HSPA9 belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. HSPA9 was localized to chromosome 5, band q31, a region that is frequently deleted in myeloid leukemias and myelodysplasia (MDS), making it a candidate tumor suppressor gene, which is consistent with the biological function of its murine homologue. Also it inhibits nuclear translocation, transcriptional activation, and control of centrosome-duplication functions of p53. Recombinant human HSPA9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock 70kDa protein 9, Mortalin, GRP75, mot-2, HSPA9B, PBP74, CSA, HSPA9, Heat shock 70kDa protein 9, Heat shock 70kDa protein 9, heat shock 70kDa protein 9 (mortalin), heat shock 70kDa protein 9B (mortalin-2), mthsp75

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

71 kDa (654aa)

Additionnal Information

GRP75, HSPA9B, Heat shock 70kDa protein 9, Mortalin, GRP75, mot-2, HSPA9B, PBP74, CSA, HSPA9, Heat shock 70kDa protein 9, Heat shock 70kDa protein 9, heat shock 70kDa protein 9 (mortalin), heat shock 70kDa protein 9B (mortalin-2), mthsp75, HSP0901-10 µg, HSP0901-20 µg, HSP0901-50 µg, HSP0901-100 µg, HSP0901-250 µg, HSP0901-500 µg, HSP0901-1 mg, HSP0901-10, HSP0901-20, HSP0901-50, HSP0901-100, HSP0901-250, HSP0901-500, HSP0901-1

References & Citations

Xie H., et al. (2000) Leukemia. 14 (12) :2128-34; ; Deocaris CC., et al. (2007) Ann N Y Acad Sci. 1119:165-75;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.5mM DTT, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_004125.3

Uniprot Accession Number

P38646

Species

Human

AA Sequence

ASEAIKGAV VGIDLGTTNS CVAVMEGKQA KVLENAEGAR TTPSVVAFTA DGERLVGMPA KRQAVTNPNN TFYATKRLIG RRYDDPEVQK DIKNVPFKIV RASNGDAWVE AHGKLYSPSQ IGAFVLMKMK ETAENYLGHT AKNAVITVPA YFNDSQRQAT KDAGQISGLN VLRVINEPTA AALAYGLDKS EDKVIAVYDL GGGTFDISIL EIQKGVFEVK STNGDTFLGG EDFDQALLRH IVKEFKRETG VDLTKDNMAL QRVREAAEKA KCELSSSVQT DINLPYLTMD SSGPKHLNMK LTRAQFEGIV TDLIRRTIAP CQKAMQDAEV SKSDIGEVIL VGGMTRMPKV QQTVQDLFGR APSKAVNPDE AVAIGAAIQG GVLAGDVTDV LLLDVTPLSL GIETLGGVFT KLINRNTTIP TKKSQVFSTA ADGQTQVEIK VCQGEREMAG DNKLLGQFTL IGIPPAPRGV PQIEVTFDID ANGIVHVSAK DKGTGREQQI VIQSSGGLSK DDIENMVKNA EKYAEEDRRK KERVEAVNMA EGIIHDTETK MEEFKDQLPA DECNKLKEEI SKMRELLARK DSETGENIRQ AASSLQQASL KLFEMAYKKM ASEREGSGSS GTGEQKEDQK EEKQ