Recombinant human HSP10/EPF protein

Heat shock protein (HSP) 10 is a member of the highly conserved group of molecular chaperons, which are necessary for efficient folding of many proteins in normal and stress conditions and have been implicated in several human diseases. HSP10 is the ~10kD mammalian equivalent to GroES of E. coli. The function of Hsp10 is to bind Hsp60 (also known as chaperonin-60), in the presence of ATP, thereby promoting a conformational change in Hsp60 and enclosing the protein substrate within the complex. ATP hydrolysis by Hsp60 destabilizes the Hsp60/ Hsp10 complex, allowing it to dissociate and release the substrate protein. Recombinant human HSP10 was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock protein 10, Chaperonin 10, CPN10, GroES, Heat shock protein 10, HSP10, Heat shock protein 10 10 kDa chaperonin, HSPE 1, HSPE1, 10 kDa heat shock protein mitochondrial, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, HSP 10, HSP10, Heat shock 10kDa protein 1 chaperonin 10

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Molecular Weight

10.9 kDa (102aa) confirmed by MALDI-TOF

Additionnal Information

HSP10, EPF, Heat shock protein 10, Chaperonin 10, CPN10, GroES, Heat shock protein 10, HSP10, Heat shock protein 10 10 kDa chaperonin, HSPE 1, HSPE1, 10 kDa heat shock protein mitochondrial, Cpn 10, Early pregnancy factor, EPF, Heat shock 10kD protein 1, Heat shock 10kD protein 1 chaperonin 10, Heat shock 10kDa protein 1, HSP 10, HSP10, Heat shock 10kDa protein 1 chaperonin 10, HSP0801-10 µg, HSP0801-20 µg, HSP0801-50 µg, HSP0801-100 µg, HSP0801-250 µg, HSP0801-500 µg, HSP0801-1 mg, HSP0801-10, HSP0801-20, HSP0801-50, HSP0801-100, HSP0801-250, HSP0801-500, HSP0801-1

References & Citations

Lin KM., et al. (2004), FASEB J. 18 (9) :1004-6; ; Hohfeld J., et al. (1994), J. Cell BIol. 126 (2) :305-15

Other References

Kaiser F, et al. Monocyte cytokine synthesis in response to extracellular cell stress proteins suggests these proteins exhibit network behaviour. (Cell Stress Chaperones. 2014) {https://pubmed.ncbi.nlm.nih.gov/23775284/}