Recombinant human HSP40/DNAJB1 protein

Human HSP40 (heat shock protein with molecular size of 40kDa) is one of the mammalian homologues of bacterial DnaJ heat shock protein and regulates cellular processes by aiding in the folding, transport and assembly. HSP40 protein contains the J-domain which controls interaction with the ATPase domain of DnaK. Recombinant human HSP40, his-tagged, is overexpressed in E. coli and purified by using the conventional column chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

40.2 kDa (360aa) confirmed by MALDI-TOF

Additionnal Information

HSP40, DNAJB1, Heat shock 40 kDa protein 1, DNAJB1, DNAJ1, HDJ1, HSPF1, HSP40 (DNAJ), Heat shock 40 kDa protein 1, Heat shock protein 40, HSP40, DnaJ protein homolog 1, HDJ-1, DnaJ homolog subfamily B member 1, Heat shock 40 kDa protein 1, radial spoke 16 homolog B (Chlamydomonas), Hsp40, Sis1, RSPH16B, HSP0701-10 µg, HSP0701-20 µg, HSP0701-50 µg, HSP0701-100 µg, HSP0701-250 µg, HSP0701-500 µg, HSP0701-1 mg, HSP0701-10, HSP0701-20, HSP0701-50, HSP0701-100, HSP0701-250, HSP0701-500, HSP0701-1

References & Citations

Hata M, Okumura K, Seto M, Ohtsuka K. (1996) . Genomics 38 (3) : 446-9; ; Manishkumar and Debashis Mitra. (2005) . Journal of Biological Chemistry 280 (48) : 40041-40050.;

Other References

Seo JH, et al. ARD1-mediated Hsp70 acetylation balances stress-induced protein refolding and degradation. (Nat Commun. 2016) {https://pubmed.ncbi.nlm.nih.gov/27708256/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_006136

Uniprot Accession Number

P25685

Species

Human

AA Sequence

MGKDYYQTLG LARGASDEEI KRAYRRQALR YHPDKNKEPG AEEKFKEIAE AYDVLSDPRK REIFDRYGEE GLKGSGPSGG SGGGANGTSF SYTFHGDPHA MFAEFFGGRN PFDTFFGQRN GEEGMDIDDP FSGFPMGMGG FTNVNFGRSR SAQEPARKKQ DPPVTHDLRV SLEEIYSGCT KKMKISHKRL NPDGKSIRNE DKILTIEVKK GWKEGTKITF PKEGDQTSNN IPADIVFVLK DKPHNIFKRD GSDVIYPARI SLREALCGCT VNVPTLDGRT IPVVFKDVIR PGMRRKVPGE GLPLPKTPEK RGDLIIEFEV IFPERIPQTS RTVLEQVLPI