Recombinant E.coli dsbC protein

Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct ones. DsbC consists of 236 amino acids containing signal peptide (1-20 amino acids) . Recombinant DsbC was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Thiol:disulfide interchange protein, Protein disulfide isomerase DsbC, Protein disulfide isomerase II, xprA

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

23.6 kDa (217aa) confirmed by MALDI-TOF

Additionnal Information

Disulfide-bond isomerase, DsbC, Disulfide-bond isomerase C, dsbC, xprA, Disulfide-bond isomerase (DsbC) E.Coli, Thiol:disulfide interchange protein dsbC, Disulfide-bond isomerase C Thiol:disulfide interchange protein dsbC ., HSP0602-10 µg, HSP0602-20 µg, HSP0602-50 µg, HSP0602-100 µg, HSP0602-250 µg, HSP0602-500 µg, HSP0602-1 mg, HSP0602-10, HSP0602-20, HSP0602-50, HSP0602-100, HSP0602-250, HSP0602-500, HSP0602-1

References & Citations

Peter W., et al. (2002) . EMBO 21, 4774-4784; ; Laura Segatori., et al. (2004) . PNAS 27, 10018-10023

Other References

Garcin EB, et al. Structural and mechanistic insights into unusual thiol disulfide oxidoreductase. (J Biol Chem. 2012) {https://pubmed.ncbi.nlm.nih.gov/22128175/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA

Scientific Category

Heat Shock Proteins

NCBI Accession Number

CAQ33205.1

Uniprot Accession Number

A0A140N4H6

Species

Human

AA Sequence

MDDAAIQQTL AKMGIKSSDI QPAPVAGMKT VLTNSGVLYI TDDGKHIIQG PMYDVSGTAP VNVTNKMLLK QLNALEKEMI VYKAPQEKHV ITVFTDITCG YCHKLHEQMA DYNALGITVR YLAFPRQGLD SDAEKEMKAI WCAKDKNKAF DDVMAGKSVA PASCDVDIAD HYVLGVQLGV SGTPAVVLSN GTLVPGYQPP KEMKEFLDEH QKMTSGK