Recombinant E.coli dsbA protein
Selected Variant
HSP0601-020
Size: 20 µg
Recombinant Disulfide Oxidoreductase (rDsbA), produced from E. coli is a periplasmic protein and thioredoxin superfamily member which introduces disulfide bonds directly into substrate proteins by donating the disulfide bond in its active-site Cys30-Pro31-His32-Cys33 to a pair of cysteines in substrate proteins. DsbA consists of 208 amino acids containing signal peptide (1-19 amino acids) . Recombinant DsbA (residues 20-208) was expressed in E. coliand purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
21.2 kDa (190aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Charbonnier, J. B. et al. (1999) . Protein Sci. 8:96-105; ; Inaba, K. et al. (2004) . J. Biol. Chem. 279:6761-6768.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM EDTA
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_418297
Uniprot Accession Number
P0AEG4
Species
Human
AA Sequence
Price
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