Recombinant Yeast HSP104 protein
Hsp104 is a molecular chaperone required for stress tolerance and for maintenance of [psi (+) ] prions in the budding yeast Saccharomyces cerevisiae. Hsp104 can protect yeast cells against high temperature and high concentration of ethanol but mutation studies have shown this protein is not required for normal growth. Hsp104 was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.
Product Specifications
Product Name Alternative
Heat shock protein 104, Protein aggregation-remodeling factor HSP104, L0948
Expression System
E.coli
Antigen Species
Yeast
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Molecular Weight
102 kDa (908aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Other References
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 2mM EDTA, 5% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_013074.1
Uniprot Accession Number
P31539
Species
Yeast
AA Sequence
Frequently Asked Questions
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