Recombinant human HSF1 protein

Heat shock factor 1 (HSF1) is a major transactivator of heat shock proteins in response to environmental changes, and it is also involved in oogenesis, spermatogenesis, and placental development. Best known for its involvement in heat shock response, Hsf1p regulates the transcription of hundreds of targets, including genes involved in protein folding, detoxification, energy generation, carbohydrate metabolism, and cell wall organization. Recombinant HSF1, fused to His tag, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Heat shock factor 1, Heat shock transcription factor 1 (HSTF), Heat shock factor 1, HSF1, Heat shock factor 1 Heat shock factor protein 1, Heat shock transcription factor 1, HSTF 1.

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 80% by SDS-PAGE

Molecular Weight

59.4 kDa (549aa)

Additionnal Information

HSF1, Heat shock factor 1, Heat shock transcription factor 1 (HSTF), Heat shock factor 1, HSF1, Heat shock factor 1 Heat shock factor protein 1, Heat shock transcription factor 1, HSTF 1., HSF0801-10 µg, HSF0801-20 µg, HSF0801-50 µg, HSF0801-100 µg, HSF0801-250 µg, HSF0801-500 µg, HSF0801-1 mg, HSF0801-10, HSF0801-20, HSF0801-50, HSF0801-100, HSF0801-250, HSF0801-500, HSF0801-1

References & Citations

Turk V, et al (1991) FEBS Lett. 285 (2) : 213-9.; ; Jarvinen M, et al, (1988) Acta Histochem. 82 (1) : 5-18

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 0.1mM PMSF

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_005517.1

Uniprot Accession Number

Q00613

Species

Human

AA Sequence

MDLPVGPGAA GPSNVPAFLT KLWTLVSDPD TDALICWSPS GNSFHVFDQG QFAKEVLPKY FKHNNMASFV RQLNMYGFRK VVHIEQGGLV KPERDDTEFQ HPCFLRGQEQ LLENIKRKVT SVSTLKSEDI KIRQDSVTKL LTDVQLMKGK QECMDSKLLA MKHENEALWR EVASLRQKHA QQQKVVNKLI QFLISLVQSN RILGVKRKIP LMLNDSGSAH SMPKYSRQFS LEHVHGSGPY SAPSPAYSSS SLYAPDAVAS SGPIISDITE LAPASPMASP GGSIDERPLS SSPLVRVKEE PPSPPQSPRV EEASPGRPSS VDTLLSPTAL IDSILRESEP APASVTALTD ARGHTDTEGR PPSPPPTSTP EKCLSVACLD KNELSDHLDA MDSNLDNLQT MLSSHGFSVD TSALLDLFSP SVTVPDMSLP DLDSSLASIQ ELLSPQEPPR PPEAENSSPD SGKQLVHYTA QPLFLLDPGS VDTGSNDLPV LFELGEGSYF SEGDGFAEDP TISLLTGSEP PKAKDPTVS