Recombinant human HSBP1 protein
Heat shock factor binding protein 1 (HSBP1) is a 76 amino acid protein that binds to heat shock factor 1 (HSF1), which is a transcription factor involved in the HS response. HSBP1 is nuclear-localized and interacts with the active trimeric state of HSF1 to negatively regulated HSF1 DNA-binding activity. This protein was overexpressed in E. coli and was purified to apparent homogeneity by using conventional column chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
Non-Tagged
Applications
SDS-PAGE
Concentration
1 mg/mL (determined by BCA assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
8.5 kDa (76aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Morimoto, R.I., et al. (1997) Essays Biochem. 32,17-29.; ; Satyal, S.H., et al. (1998) Genes Dev. 12 (13) 1962-1974.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing, 50 mM NaCl, 20% glycerol
Scientific Category
Heat Shock Proteins
NCBI Accession Number
NP_001528.1
Uniprot Accession Number
O75506
Species
Human
AA Sequence
MAETDPKTVQ DLTSVVQTLL QQMQDKFQTM SDQIIGRIDD MSSRIDDLEK NIADLMTQAG VEELESENKI PATQKS
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