Recombinant human HMG-1/HMGB1 protein

High mobility group box1 protein (HMGB1) is a very abundant chromatin-binding protein residing in the eukaryotic cell nucleus and acting in the assembly of nucleoprotein complexes. Inside the cell, HMGB1 binds to DNA and has a role in transcriptional regulation. Outside the cell, HMGB1 acts as a cytokine and has activities that resemble those of tumor necrosis factor. Recombinant human HMGB1 was expressed in insect cells and purified by conventional chromatography techniques.

Product Specifications

Product Name Alternative

Sulfoglucuronyl carbohydrate binding protein, SBP-1, HMGB1, HMG3, HMG1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

25 kDa (223aa) confirmed by MALDI-TOF

Additionnal Information

Sulfoglucuronyl carbohydrate binding protein, SBP-1, SBP1, SBP 1, HMGB1, HMGB 1, HMG3, HMG1, HMG 1, High-mobility group box1, High-mobility group (nonhistone chromosomal) protein 1, DKFZp686A04236, Amphoterin, HMG 3, High mobility group box1, High mobility group (nonhistone chromosomal) protein 1, High mobility group protein 1, HMG0801-10 µg, HMG0801-20 µg, HMG0801-50 µg, HMG0801-100 µg, HMG0801-250 µg, HMG0801-500 µg, HMG0801-1 mg, HMG0801-010, HMG0801-020, HMG0801-050, HMG0801-100, HMG0801-250, HMG0801-500, HMG0801-01M

References & Citations

Palumbo R J., et al. (2004) Cell Biol. 164 (3) :441-9.; ; Andersson u., et al (2002) Leukoc Biol. 72 (6) :1084-91.;

Other References

Min HJ et al., Chaperone-like Activity of High-Mobility Group Box 1 Protein and Its Role in Reducing the Formation of Polyglutamine Aggregates. (J Immunol. 2013) {https://www.ncbi.nlm.nih.gov/pubmed/23303669}; ; Slean MM et al., Interconverting Conformations of Slipped-DNA Junctions Formed by Trinucleotide Repeats Affect Repair Outcome. (Biochemistry. 2013) {https://www.ncbi.nlm.nih.gov/pubmed/23339280}; ; Takahashi T et al., Bone-Targeting Endogenous Secretory Receptor for Advanced Glycation End Products Rescues Rheumatoid Arthritis. (Mol Med. 2013) {https://www.ncbi.nlm.nih.gov/pubmed/23821362}; ; Roy A et al., Mast cell chymase degrades the alarmins heat shock protein 70, biglycan, HMGB1, and interleukin-33 (IL-33) and limits danger-induced inflammation. (J Biol Chem. 2014) {https://pubmed.ncbi.nlm.nih.gov/24257755/}; ; Sugimoto K et al., Receptor for Advanced Glycation End Products - Membrane Type1 Matrix Metalloproteinase Axis Regulates Tissue Factor Expression via RhoA and Rac1 Activation in High-Mobility Group Box-1 Stimulated Endothelial Cells. (PLoS One. 2014) {https://pubmed.ncbi.nlm.nih.gov/25490770/}; ; Hernández-Pando R et al. The Role of High Mobility Group Box 1 Protein (HMGB1) in the Immunopathology of Experimental Pulmonary Tuberculosis. (PLoS One. 2015) {https://pubmed.ncbi.nlm.nih.gov/26201072/}; ; Ito M et al., High mobility group box 1 enhances hyperthermia-induced seizures and secondary epilepsy associated with prolonged hyperthermia-induced seizures in developing rats. (Metab Brain Dis. 2017) {https://pubmed.ncbi.nlm.nih.gov/28879430/}; ; Jang J et al., Recombinant RAGE antagonist peptide promotes alveolar epithelial cell regeneration via the RAGE/MAPKs/MMP2 pathway in emphysema. (Biochem Pharmacol. 2025) {https://pubmed.ncbi.nlm.nih.gov/39608502/}