Recombinant human HIP/ST13 protein

Hip (Hsc70-interacting protein), also known as ST13, is a co-chaperone to the major heat shock proteins, Hsp70 and Hsp90, and appears in early receptor complexes. Through mutual binding to both Hsp70 and Hsp90, Hip functions as an adaptor that can integrate Hsp70 and Hsp90 interactions. Also, Hip has been shown to be involved in the assembly process of glucocorticoid receptor, which requires the assistance of multiple molecular chaperones. Recombinant human Hip was expressed in E. coli and purified by using conventional chromatography

Product Specifications

Product Name Alternative

Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Molecular Weight

41.3 kDa (369aa) confirmed by MALDI-TOF

Additionnal Information

Hip, ST13, Hsc70-interacting protein, ST13, HOP, SNC6, FAM10A1, Hsc70-interacting protein, HIP0905-10 µg, HIP0905-20 µg, HIP0905-50 µg, HIP0905-100 µg, HIP0905-250 µg, HIP0905-500 µg, HIP0905-1 mg, HIP0905-10, HIP0905-20, HIP0905-50, HIP0905-100, HIP0905-250, HIP0905-500, HIP0905-1

References & Citations

Nelson GM., et al. (2004) . Mol Endocrinol. 18 (7) :1620-30; ; Johnson BD., et al. (1998) . J Biol chem. 273 (6) :3679-86;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M NaCl, 10% glycerol

Scientific Category

Heat Shock Proteins

NCBI Accession Number

NP_003923

Uniprot Accession Number

P50502

Species

Human

AA Sequence

MDPRKVNELR AFVKMCKQDP SVLHTEEMRF LREWVESMGG KVPPATQKAK SEENTKEEKP DSKKVEEDLK ADEPSSEESD LEIDKEGVIE PDTDAPQEMG DENAEITEEM MDQANDKKVA AIEALNDGEL QKAIDLFTDA IKLNPRLAIL YAKRASVFVK LQKPNAAIRD CDRAIEINPD SAQPYKWRGK AHRLLGHWEE AAHDLALACK LDYDEDASAM LKEVQPRAQK IAEHRRKYER KREEREIKER IERVKKAREE HERAQREEEA RRQSGAQYGS FPGGFPGGMP GNFPGGMPGM GGGMPGMAGM PGLNEILSDP EVLAAMQDPE VMVAFQDVAQ NPANMSKYQS NPKVMNLISK LSAKFGGQA